For many uses, biometric systems have gained considerable attention. Iris identification was One of the most powerful sophisticated biometrical techniques for effective and confident authentication. The current iris identification system offers accurate and reliable results based on near-infrared light (NIR) images when images are taken in a restricted area with fixed- distance user cooperation. However, for the color eye images obtained under visible wavelength (VW) without collaboration among the users, the efficiency of iris recognition degrades because of noise such as eye blurring images, eye lashing, occlusion, and reflection. This work aims to use the Gray-Level Co-occurrence Matrix (GLCM) to retrieve the iris's characteristics in both NIR iris images and visible spectrum. GLCM is second-order Statistical-Based Methods for Texture Analysis. The GLCM- based extraction technology was applied after the preprocessing method to extract the pure iris region's characteristics. The Energy, Entropy, Correlation, Homogeneity, and Contrast collection of second-order statistical features are determined from the generated co-occurrence matrix, Stored as a vector for numerical features. This approach is used and evaluated on the CASIA v1and ITTD v1 databases as NIR iris image and UBIRIS v1 as a color image. The results showed a high accuracy rate (99.2 %) on CASIA v1, (99.4) on ITTD v1, and (87%) on UBIRIS v1 evaluated by comparing to the other methods.