The operational variables of Proton Exchange Membrane Fuel Cell (PEMFC) such as cell temperature, hydrogen gas pressures, and oxygen gas pressures are highly effect on the power generation from the PEMFC. Therefore, the Maximum Power Point Tracker (MPPT) should be used to increase the efficiency of PEMFC at different operational variables. Unfortunately, the majority of conventional MPPT algorithms will cause PEMFC damage and power loss by producing steady-state oscillations. This paper focuses on enhancing the efficiency of the Proton Exchange Membrane Fuel Cell through the utilization of advanced control methods: Grey Wolf Optimizer (GWO), GWO with a PID controller and perturbation and observation (P&O) techniques. The objective is to effectively manage power output by pinpointing the maximum power point and reducing stable oscillations. The study evaluates these methods in swiftly changing operational scenarios and compares their performances. The obtained results show that the GWO with a PID controller increase generation power.